PDBe 2dv6

X-ray diffraction
2.2Å resolution

Crystal structure of nitrite reductase from Hyphomicrobium denitrificans

Released:
Source organism: Hyphomicrobium denitrificans
Primary publication:
Structure and function of a hexameric copper-containing nitrite reductase.
Proc. Natl. Acad. Sci. U.S.A. 104 4315-20 (2007)
PMID: 17360521

Function and Biology Details

Reaction catalysed:
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 447 amino acids
Theoretical weight: 48.22 KDa
Source organism: Hyphomicrobium denitrificans
UniProt:
  • Canonical: Q8KKH4 (Residues: 40-486; Coverage: 97%)
Gene name: nirK
Sequence domains: Multicopper oxidase
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P41
Unit cell:
a: 162.551Å b: 162.551Å c: 148.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.152 0.165