PDBe 2den

Solution NMR

Solution Structure of the Ubiquitin-Associated Domain of Human BMSC-UbP and its Complex with Ubiquitin

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-like protein 7 Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 11.91 KDa
Source organism: Streptococcus sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96S82 (Residues: 336-380; Coverage: 12%)
Gene names: BMSCUBP, SB132, UBL7
Structure domains: DNA helicase RuvA subunit, C-terminal domain
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 30%
Refinement method: molecular dynamics
Chemical shifts: BMR6988  
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)