PDBe 2ddt

X-ray diffraction
1.8Å resolution

Crystal structure of sphingomyelinase from Bacillus cereus with magnesium ion

Released:

Function and Biology Details

Reaction catalysed:
Sphingomyelin + H(2)O = N-acylsphingosine + phosphocholine
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sphingomyelinase C Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 34.34 KDa
Source organism: Bacillus cereus
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P11889 (Residues: 28-333; Coverage: 100%)
Gene name: sph
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P1
Unit cell:
a: 50.844Å b: 50.893Å c: 59.506Å
α: 81.87° β: 81.84° γ: 79.68°
R-values:
R R work R free
0.195 0.193 0.23
Expression system: Bacillus subtilis