PDBe 2czc

X-ray diffraction
2Å resolution

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Pyrococcus horikoshii OT3

Released:
Source organism: Pyrococcus horikoshii OT3
Entry authors: Ito K, Arai R, Kamo-Uchikubo T, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 334 amino acids
Theoretical weight: 37.31 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59494 (Residues: 1-334; Coverage: 100%)
Gene names: PH1830, gap
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 67.693Å b: 127.192Å c: 161.454Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.241
Expression system: Escherichia coli