PDBe 2cnx

X-ray diffraction
2.1Å resolution

WDR5 and Histone H3 Lysine 4 dimethyl complex at 2.1 angstrom

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 34.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61964 (Residues: 20-334; Coverage: 94%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone H3.1 Chain: P
Molecule details ›
Chain: P
Length: 5 amino acids
Theoretical weight: 632 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-6; Coverage: 4%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 100.249Å b: 46.753Å c: 64.789Å
α: 90° β: 107.08° γ: 90°
R-values:
R R work R free
0.192 0.192 0.223
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided