PDBe 2cia

X-ray diffraction
1.45Å resolution

human nck2 sh2-domain in complex with a decaphosphopeptide from translocated intimin receptor (tir) of epec

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cytoplasmic protein NCK2 Chain: A
Molecule details ›
Chain: A
Length: 102 amino acids
Theoretical weight: 11.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O43639 (Residues: 282-380; Coverage: 26%)
Gene names: GRB4, NCK2
Sequence domains: SH2 domain
Structure domains: SH2 domain
Translocated intimin receptor Tir Chain: L
Molecule details ›
Chain: L
Length: 11 amino acids
Theoretical weight: 1.24 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: B7UM99 (Residues: 472-481; Coverage: 2%)
Gene names: E2348C_3941, espE, tir

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 31.244Å b: 52.188Å c: 58.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.17
Expression systems:
  • Escherichia coli BL21
  • Not provided