PDBe 2ci9

X-ray diffraction
1.5Å resolution

Nck1 SH2-domain in complex with a dodecaphosphopeptide from EPEC protein Tir

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cytoplasmic protein NCK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 102 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P16333 (Residues: 281-377; Coverage: 26%)
Gene names: NCK, NCK1
Sequence domains: SH2 domain
Structure domains: SH2 domain
Translocated intimin receptor Tir Chains: L, M
Molecule details ›
Chains: L, M
Length: 12 amino acids
Theoretical weight: 1.47 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: B7UM99 (Residues: 470-481; Coverage: 2%)
Gene names: E2348C_3941, espE, tir

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 55.092Å b: 60.518Å c: 65.061Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.235
Expression systems:
  • Escherichia coli BL21
  • Not provided