PDBe 2cg5

X-ray diffraction
2.7Å resolution

Structure of aminoadipate-semialdehyde dehydrogenase- phosphopantetheinyl transferase in complex with cytosolic acyl carrier protein and coenzyme A

Released:
Source organism: Homo sapiens

Function and Biology Details

Reactions catalysed:
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+)
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate
An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase Chain: A
Molecule details ›
Chain: A
Length: 319 amino acids
Theoretical weight: 37.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NRN7 (Residues: 14-309; Coverage: 96%)
Gene names: AASDHPPT, CGI-80, HAH-P, HSPC223, x0005
Sequence domains: 4'-phosphopantetheinyl transferase superfamily
Structure domains: 4'-phosphopantetheinyl transferase domain
Fatty acid synthase Chain: B
Molecule details ›
Chain: B
Length: 91 amino acids
Theoretical weight: 10.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49327 (Residues: 2119-2207; Coverage: 4%)
Gene names: FAS, FASN
Sequence domains: Phosphopantetheine attachment site
Structure domains: ACP-like

Ligands and Environments


Cofactor: Ligand COA 1 x COA
3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P3221
Unit cell:
a: 69.361Å b: 69.361Å c: 184.715Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli