PDBe 2bx2

X-ray diffraction
2.85Å resolution

Catalytic domain of E. coli RNase E

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease E Chain: L
Molecule details ›
Chain: L
Length: 517 amino acids
Theoretical weight: 58.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21513 (Residues: 1-510; Coverage: 48%)
Gene names: JW1071, ams, b1084, hmp1, rne
Sequence domains:
RNA (5'-R(*UP*UP*UP*AP*CP*AP*GP*UP*AP*UP*UP* UP*GP*UP*U)-3') Chain: R
Molecule details ›
Chain: R
Length: 15 nucleotides
Theoretical weight: 4.69 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6222
Unit cell:
a: 195.838Å b: 195.838Å c: 143.569Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.23 0.257
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided