PDBe 2bnr

X-ray diffraction
1.9Å resolution

Structural and kinetic basis for heightened immunogenicity of T cell vaccines

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-300; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Cancer/testis antigen 1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P78358 (Residues: 157-165; Coverage: 5%)
Gene names: CTAG, CTAG1, CTAG1A, CTAG1B, ESO1, LAGE2, LAGE2A, LAGE2B
T cell receptor alpha constant; T cell receptor alpha variable 21 Chain: D
Molecule details ›
Chain: D
Length: 203 amino acids
Theoretical weight: 22.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01848 (Residues: 1-89; Coverage: 64%)
  • Canonical: A0A0B4J279 (Residues: 21-112; Coverage: 99%)
  • nullnull
Gene names: TCRA, TRAC, TRAV21
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
T cell receptor beta constant 1; T cell receptor beta variable 6-5 Chain: E
Molecule details ›
Chain: E
Length: 241 amino acids
Theoretical weight: 27.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K0K1A5 (Residues: 22-114; Coverage: 100%)
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
  • nullnull
Gene names: TRBC1, TRBV6-5
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 72.563Å b: 62.49Å c: 116.955Å
α: 90° β: 105.34° γ: 90°
R-values:
R R work R free
0.231 0.231 not available
Expression systems:
  • Escherichia coli
  • Not provided