PDBe 2bkr

X-ray diffraction
1.9Å resolution

NEDD8 NEDP1 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sentrin-specific protease 8 Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 24.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q96LD8 (Residues: 1-212; Coverage: 100%)
Gene names: DEN1, FKSG8, NEDP1, PRSC2, SENP8
Sequence domains: Ulp1 protease family, C-terminal catalytic domain
Structure domains: Adenoviral Proteinase; Chain A
NEDD8 Chain: B
Molecule details ›
Chain: B
Length: 77 amino acids
Theoretical weight: 8.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q15843 (Residues: 1-76; Coverage: 94%)
Gene name: NEDD8
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 54.638Å b: 74.226Å c: 79.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.209
Expression system: Escherichia coli BL21(DE3)