PDBe 2bel

X-ray diffraction
2.11Å resolution

Structure of human 11-beta-hydroxysteroid dehydrogenase in complex with NADP and carbenoxolone

Released:
Source organism: Homo sapiens
Entry authors: Kavanagh K, Wu X, Svensson S, Elleby B, von Delft F, Debreczeni JE, Sharma S, Bray J, Edwards A, Arrowsmith C, Sundstrom M, Abrahmsen L, Oppermann U

Function and Biology Details

Reaction catalysed:
An 11-beta-hydroxysteroid + NADP(+) = an 11-oxosteroid + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Corticosteroid 11-beta-dehydrogenase isozyme 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 283 amino acids
Theoretical weight: 31.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28845 (Residues: 20-284; Coverage: 91%)
Gene names: HSD11, HSD11B1, HSD11L, SDR26C1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 160.162Å b: 112.984Å c: 66.326Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.246
Expression system: Escherichia coli BL21(DE3)