PDBe 2b4s

X-ray diffraction
2.3Å resolution

Crystal structure of a complex between PTP1B and the insulin receptor tyrosine kinase

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin receptor subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 306 amino acids
Theoretical weight: 35.03 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P06213 (Residues: 1005-1310; Coverage: 23%)
Gene name: INSR
Sequence domains: Protein tyrosine kinase
Structure domains:
Tyrosine-protein phosphatase non-receptor type 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 298 amino acids
Theoretical weight: 34.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18031 (Residues: 1-298; Coverage: 69%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 87.472Å b: 88.165Å c: 178.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.239
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)