PDBe 2b3g

X-ray diffraction
1.6Å resolution

p53N (fragment 33-60) bound to RPA70N

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Replication protein A 70 kDa DNA-binding subunit, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27694 (Residues: 1-120; Coverage: 20%)
Gene names: REPA1, RPA1, RPA70
Sequence domains: Replication factor-A protein 1, N-terminal domain
Structure domains: Nucleic acid-binding proteins
Cellular tumor antigen p53 Chain: B
Molecule details ›
Chain: B
Length: 28 amino acids
Theoretical weight: 3.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 33-60; Coverage: 7%)
Gene names: P53, TP53
Sequence domains: Transactivation domain 2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 49.46Å b: 50.38Å c: 52.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.239
Expression system: Escherichia coli BL21(DE3)