PDBe 2aze

X-ray diffraction
2.55Å resolution

Structure of the Rb C-terminal domain bound to an E2F1-DP1 heterodimer

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Transcription factor Dp-1 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q14186 (Residues: 199-350; Coverage: 37%)
Gene names: DP1, TFDP1
Sequence domains: Transcription factor DP
Structure domains: Butyryl-CoA Dehydrogenase, subunit A; domain 3
Transcription factor E2F1 Chain: B
Molecule details ›
Chain: B
Length: 106 amino acids
Theoretical weight: 11.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q01094 (Residues: 200-301; Coverage: 23%)
Gene names: E2F1, RBBP3
Sequence domains: E2F transcription factor CC-MB domain
Retinoblastoma-associated protein Chain: C
Molecule details ›
Chain: C
Length: 46 amino acids
Theoretical weight: 5.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P06400 (Residues: 829-874; Coverage: 5%)
Gene name: RB1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C, NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 146.8Å b: 168.6Å c: 48.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.262
Expression system: Escherichia coli