› 2axf

X-ray diffraction
1.8Å resolution

The Immunogenicity of a Viral Cytotoxic T Cell Epitope is controlled by its MHC-bound Conformation

Released:
DOI: 10.2210/pdb2axf/pdb
PDB entry 2axf coloured by chain, front view.
PDB entry 2axf coloured by chain, side view.
PDB entry 2axf coloured by chain, top view.
Source organisms:
Primary publication:
The immunogenicity of a viral cytotoxic T cell epitope is controlled by its MHC-bound conformation.
Tynan FE, Elhassen D, Purcell AW, Burrows JM, Borg NA, Miles JJ, Williamson NA, Green KJ, Tellam J, Kjer-Nielsen L, McCluskey J, Rossjohn J, Burrows SR
J Exp Med 202 1249-60 (2005)
PMID: 16275762

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135131 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01889 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Lytic switch protein BZLF1 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.06 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P03206 (Residues: 77-86; Coverage: 4%)
Gene name: BZLF1

Ligands and Environments

1 bound ligand:
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 50.825Å b: 81.528Å c: 110.732Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.234
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

16 review citations

More than one reason to rethink the use of peptides in vaccine design.
Purcell et al. (2007)
15 more

3 mentions without citation

The immunogenicity of a viral cytotoxic T cell epitope is controlled by its MHC-bound conformation.
Tynan et al. (2005)
2 more