PDBe 2avf

X-ray diffraction
2.6Å resolution

Crystal Structure of C-terminal Desundecapeptide Nitrite Reductase from Achromobacter cycloclastes

Released:

Function and Biology Details

Reaction catalysed:
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 329 amino acids
Theoretical weight: 35.91 KDa
Source organism: Achromobacter cycloclastes
Expression system: Escherichia coli
UniProt:
  • Canonical: P25006 (Residues: 39-367; Coverage: 97%)
Gene name: nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P21
Unit cell:
a: 85.783Å b: 111.072Å c: 122.884Å
α: 90° β: 101.67° γ: 90°
R-values:
R R work R free
0.182 0.182 0.217
Expression system: Escherichia coli