PDBe 2auh

X-ray diffraction
3.2Å resolution

Crystal structure of the Grb14 BPS region in complex with the insulin receptor tyrosine kinase

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin receptor subunit beta Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 35.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06213 (Residues: 1005-1310; Coverage: 23%)
Gene name: INSR
Sequence domains: Protein tyrosine kinase
Structure domains:
Growth factor receptor-bound protein 14 Chain: B
Molecule details ›
Chain: B
Length: 59 amino acids
Theoretical weight: 6.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14449 (Residues: 361-419; Coverage: 11%)
Gene name: GRB14
Sequence domains: BPS (Between PH and SH2)

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P62
Unit cell:
a: 127.182Å b: 127.182Å c: 65.925Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.223 0.254
Expression system: Escherichia coli