PDBe 2as5

X-ray diffraction
2.7Å resolution

Structure of the DNA binding domains of NFAT and FOXP2 bound specifically to DNA.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Nuclear factor of activated T-cells, cytoplasmic 2 Chains: M, N
Molecule details ›
Chains: M, N
Length: 287 amino acids
Theoretical weight: 32.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13469 (Residues: 392-678; Coverage: 31%)
Gene names: NFAT1, NFATC2, NFATP
Sequence domains: Rel homology DNA-binding domain
Structure domains:
Forkhead box protein P2 Chains: F, G
Molecule details ›
Chains: F, G
Length: 93 amino acids
Theoretical weight: 11.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15409 (Residues: 502-594; Coverage: 13%)
Gene names: CAGH44, FOXP2, TNRC10
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(TP*TP*AP*GP*GP*AP*AP*AP*AP*TP*TP*TP*GP*TP*TP*TP*CP*AP*TP*AP*GP*)-3' Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
5'-D(AP*AP*CP*TP*AP*TP*GP*AP*AP*AP*CP*AP*AP*AP*TP*TP*TP*TP*CP*CP*TP*)-3' Chains: B, D
Molecule details ›
Chains: B, D
Length: 21 nucleotides
Theoretical weight: 6.39 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 65.455Å b: 157.447Å c: 67.666Å
α: 90° β: 118.67° γ: 90°
R-values:
R R work R free
0.238 0.238 0.287
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli