PDBe 2any

X-ray diffraction
1.4Å resolution

Expression, Crystallization and the Three-dimensional Structure of the Catalytic Domain of Human Plasma Kallikrein: Implications for Structure-Based Design of Protease Inhibitors


Function and Biology Details

Reaction catalysed:
Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Plasma kallikrein light chain Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 27.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: P03952 (Residues: 391-631; Coverage: 39%)
Gene names: KLK3, KLKB1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 55.187Å b: 57.359Å c: 79.846Å
α: 90° β: 90° γ: 90°
R R work R free
0.19 0.188 0.213
Expression system: Spodoptera frugiperda