PDBe 2anw

X-ray diffraction
1.85Å resolution

Expression, crystallization and three-dimensional structure of the catalytic domain of human plasma kallikrein: Implications for structure-based design of protease inhibitors

Released:

Function and Biology Details

Reaction catalysed:
Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasma kallikrein light chain Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 27.1 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P03952 (Residues: 391-631; Coverage: 39%)
Gene names: KLK3, KLKB1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21212
Unit cell:
a: 79.886Å b: 63.19Å c: 50.313Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.283
Expression system: Spodoptera frugiperda