PDBe 2af3

X-ray diffraction
2.6Å resolution

Phosphotransacetylase from Methanosarcina thermophila soaked with Coenzyme A

Released:

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + phosphate = CoA + acetyl phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate acetyltransferase Chains: C, D
Molecule details ›
Chains: C, D
Length: 333 amino acids
Theoretical weight: 35.26 KDa
Source organism: Methanosarcina thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38503 (Residues: 1-333; Coverage: 100%)
Gene name: pta
Sequence domains: Phosphate acetyl/butaryl transferase
Structure domains:

Ligands and Environments


Cofactor: Ligand COA 3 x COA
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: I41
Unit cell:
a: 114.469Å b: 114.469Å c: 127.832Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.215 0.29
Expression system: Escherichia coli BL21(DE3)