PDBe 1zzn

X-ray diffraction
3.37Å resolution

Crystal structure of a group I intron/two exon complex that includes all catalytic metal ion ligands.

Released:
Source organism: Homo sapiens
Primary publication:
Structural evidence for a two-metal-ion mechanism of group I intron splicing.
Science 309 1587-90 (2005)
PMID: 16141079

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct RNA molecules
Macromolecules (4 distinct):
U1 small nuclear ribonucleoprotein A Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 11.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09012 (Residues: 1-98; Coverage: 35%)
Gene name: SNRPA
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
197-MER Chain: B
Molecule details ›
Chain: B
Length: 197 nucleotides
Theoretical weight: 64.06 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-R(*AP*AP*GP*CP*CP*AP*CP*AP*CP*AP*AP*AP*CP*CP*AP*GP*AP*CP*GP*GP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 22 nucleotides
Theoretical weight: 7.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-R(*CP*AP*(5MU))-3' Chain: D
Molecule details ›
Chain: D
Length: 3 nucleotides
Theoretical weight: 910 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:

3 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P4122
Unit cell:
a: 108.466Å b: 108.466Å c: 246.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.269 0.307
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided