PDBe 1zud

X-ray diffraction
1.98Å resolution

Structure of ThiS-ThiF protein complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sulfur carrier protein ThiS adenylyltransferase Chains: 1, 3
Molecule details ›
Chains: 1, 3
Length: 251 amino acids
Theoretical weight: 26.99 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P30138 (Residues: 1-251; Coverage: 100%)
Gene names: JW3956, b3992, thiF
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain
Sulfur carrier protein ThiS Chains: 2, 4
Molecule details ›
Chains: 2, 4
Length: 66 amino acids
Theoretical weight: 7.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: O32583 (Residues: 1-66; Coverage: 100%)
Gene names: JW3955, b4407, thiG1, thiS
Sequence domains: ThiS family
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P212121
Unit cell:
a: 49.505Å b: 111.171Å c: 114.153Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.174 0.229
Expression system: Escherichia coli