PDBe 1zr4

X-ray diffraction
3.4Å resolution

Structure of a Synaptic gamma-delta Resolvase Tetramer Covalently linked to two Cleaved DNAs

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 16-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Transposon gamma-delta resolvase Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 183 amino acids
Theoretical weight: 20.35 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03012 (Residues: 1-183; Coverage: 100%)
Gene names: ECOK12F009, tnpR
Sequence domains:
Structure domains:
AAA Chains: I, O, W, Z
Molecule details ›
Chains: I, O, W, Z
Length: 3 nucleotides
Theoretical weight: 895 Da
Source organism: Synthetic construct
Expression system: Not provided
TTATCGGACACTG Chains: K, N, V, Y
Molecule details ›
Chains: K, N, V, Y
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Synthetic construct
Expression system: Not provided
TCAGTGTCCGATAATTTAT Chains: J, M, U, X
Molecule details ›
Chains: J, M, U, X
Length: 19 nucleotides
Theoretical weight: 5.81 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 119.158Å b: 127.29Å c: 140.419Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.264 0.295
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided