PDBe 1zq3

Solution NMR

NMR Solution Structure of the Bicoid Homeodomain Bound to the Consensus DNA Binding Site TAATCC

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeotic protein bicoid Chain: P
Molecule details ›
Chain: P
Length: 68 amino acids
Theoretical weight: 7.96 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P09081 (Residues: 97-163; Coverage: 14%)
Gene names: CG1034, bcd
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
5'-D(*CP*GP*GP*GP*GP*AP*TP*TP*AP*GP*AP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4.06 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*GP*CP*TP*CP*TP*AP*AP*TP*CP*CP*CP*CP*G)-3' Chain: A
Molecule details ›
Chain: A
Length: 13 nucleotides
Theoretical weight: 3.89 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: protein structure - fast torsion angle dynamics algorithm (CYANA2.0), DNA structure - NUCGEN and simulated annealing with NMR-derived energy restraints (AMBER7.0), protein-DNA complex - simulated annealing with NMR-derived energy restraints (AMBER7.0), protein-DNA complex refinement - explicit solvent MD simulations (AMBER7.0)
Expression systems:
  • Escherichia coli
  • Not provided