PDBe 1zp3

X-ray diffraction
1.85Å resolution

E. coli Methylenetetrahydrofolate Reductase (oxidized)

Released:

Function and Biology Details

Reaction catalysed:
5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5,10-methylenetetrahydrofolate reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 304 amino acids
Theoretical weight: 34.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEZ1 (Residues: 1-296; Coverage: 100%)
Gene names: JW3913, b3941, metF
Sequence domains: Methylenetetrahydrofolate reductase
Structure domains: TIM Barrel

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 103.236Å b: 127.789Å c: 97.703Å
α: 90° β: 121.55° γ: 90°
R-values:
R R work R free
0.229 0.214 0.237
Expression system: Escherichia coli