PDBe 1zot

X-ray diffraction
2.2Å resolution

crystal structure analysis of the CyaA/C-Cam with PMEAPP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-2 Chain: B
Molecule details ›
Chain: B
Length: 69 amino acids
Theoretical weight: 7.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP24 (Residues: 80-148; Coverage: 46%)
Gene names: CALM2, CAM2, CAMB
Structure domains: EF-hand
Calmodulin-sensitive adenylate cyclase Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 38.69 KDa
Source organism: Bordetella pertussis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DKX7 (Residues: 7-364; Coverage: 21%)
Gene names: BP0760, cya, cyaA
Sequence domains: Anthrax toxin LF subunit
Structure domains: Adenylylcyclase toxin superfamily

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P41212
Unit cell:
a: 79.674Å b: 79.674Å c: 139.339Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.252 0.291
Expression system: Escherichia coli