› 1zny

X-ray diffraction
2.3Å resolution

Crystal Structure Of Mycobacterium tuberculosis Guanylate Kinase In Complex With GDP

Released:
DOI: 10.2210/pdb1zny/pdb
PDB entry 1zny coloured by chain, front view.
PDB entry 1zny coloured by chain, side view.
PDB entry 1zny coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Unique GMP-binding site in Mycobacterium tuberculosis guanosine monophosphate kinase.
Hible G, Christova P, Renault L, Seclaman E, Thompson A, Girard E, Munier-Lehmann H, Cherfils J
Proteins 62 489-500 (2006)
PMID: 16288457

Function and Biology Details

Reaction catalysed: 
ATP + GMP = ADP + GDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161738 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate kinase Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 21.99 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WKE9 (Residues: 2-208; Coverage: 100%)
Gene names: MTCY21B4.06, Rv1389, gmk
Sequence domains: Guanylate kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I23
Unit cell:
a: 113.046Å b: 113.046Å c: 113.046Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.236
Expression system: Escherichia coli

Quick links

Citations

16 citation in other articles

Crystal structure and nucleotide binding of the Thermus thermophilus RNA helicase Hera N-terminal domain.
Rudolph et al. (2006)
15 more

1 mention without citation

Strain analysis of protein structures and low dimensionality of mechanical allosteric couplings.
Mitchell et al. (2016)