PDBe 1znv

X-ray diffraction
2Å resolution

How a His-metal finger endonuclease ColE7 binds and cleaves DNA with a transition metal ion cofactor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E7 Chains: B, D
Molecule details ›
Chains: B, D
Length: 134 amino acids
Theoretical weight: 15.4 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47112 (Residues: 444-576; Coverage: 23%)
Gene names: cea, colE7
Structure domains: Colicin e7 immunity protein. Chain B
Colicin-E7 immunity protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 93 amino acids
Theoretical weight: 10.74 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03708 (Residues: 1-87; Coverage: 100%)
Gene names: ceiE7, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P21212
Unit cell:
a: 119.021Å b: 62.882Å c: 74.769Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.235
Expression system: Escherichia coli