PDBe 1znb

X-ray diffraction
1.85Å resolution

METALLO-BETA-LACTAMASE

Released:

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 25.27 KDa
Source organism: Bacteroides fragilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P25910 (Residues: 18-249; Coverage: 100%)
Gene names: ccrA, cfiA
Structure domains: Metallo-beta-lactamase, chain A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, NSLS BEAMLINE X12C
Spacegroup: P43212
Unit cell:
a: 78.298Å b: 78.298Å c: 140.916Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.167 0.241
Expression system: Escherichia coli BL21