PDBe 1zme

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF PUT3/DNA COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Proline utilization trans-activator Chains: C, D
Molecule details ›
Chains: C, D
Length: 70 amino acids
Theoretical weight: 7.96 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P25502 (Residues: 31-100; Coverage: 7%)
Gene names: PUT3, YKL015W
Sequence domains: Fungal Zn(2)-Cys(6) binuclear cluster domain
Structure domains: CD2-Gal4
DNA (5'-D(*AP*CP*GP*GP*GP*AP*AP*GP*CP*CP*AP*AP*CP*TP*CP*CP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 17 nucleotides
Theoretical weight: 5.21 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
DNA (5'-D(*AP*CP*GP*GP*AP*GP*(5IU)P*TP*GP*GP*CP*TP*(5IU)P*CP*CP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.44 KDa
Source organism: Unidentified
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6
Unit cell:
a: 121.65Å b: 121.65Å c: 39.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.208 0.292
Expression systems:
  • Escherichia coli
  • Not provided