PDBe 1zkm

X-ray diffraction
2.95Å resolution

Structural Analysis of Escherichia Coli ThiF

Released:
Source organism: Escherichia coli
Primary publication:
Structural analysis of Escherichia coli ThiF.
J. Mol. Biol. 349 774-86 (2005)
PMID: 15896804

Function and Biology Details

Reaction catalysed:
ATP + [ThiS] = diphosphate + adenylyl-[ThiS]
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfur carrier protein ThiS adenylyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 253 amino acids
Theoretical weight: 27.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30138 (Residues: 1-251; Coverage: 100%)
Gene names: JW3956, b3992, thiF
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: F4132
Unit cell:
a: 360.168Å b: 360.168Å c: 360.168Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.28
Expression system: Escherichia coli BL21(DE3)