› 1zie

X-ray diffraction
1.44Å resolution

Hydrogenated gammaE crystallin in D2O solvent

Released:
DOI: 10.2210/pdb1zie/pdb
PDB entry 1zie coloured by chain, front view.
PDB entry 1zie coloured by chain, side view.
PDB entry 1zie coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O.
Artero JB, Härtlein M, McSweeney S, Timmins P
Acta Crystallogr D Biol Crystallogr 61 1541-9 (2005)
PMID: 16239733

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135885 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-crystallin E Chain: A
Molecule details ›
Chain: A
Length: 173 amino acids
Theoretical weight: 21.16 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02528 (Residues: 2-174; Coverage: 99%)
Gene name: Cryge
Sequence domains: Beta/Gamma crystallin
Structure domains: Crystallins

Ligands and Environments

2 bound ligands:
Ligand ACT 2 x ACT
Ligand DOD 202 x DOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 37.63Å b: 43.42Å c: 101.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.167 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Neutron protein crystallography: A complementary tool for locating hydrogens in proteins.
O'Dell et al. (2016)
1 more

2 mentions without citation

Biophysical properties of gammaC-crystallin in human and mouse eye lens: the role of molecular dipoles.
Purkiss et al. (2007)
1 more