› 1zh4

X-ray diffraction
2.2Å resolution

Crystal Structure Of The Mg+2/BeF3-Bound Receiver Domain Of Kdp Potassium Transport System Response Regulator KdpE

Released:
DOI: 10.2210/pdb1zh4/pdb
PDB entry 1zh4 coloured by chain, front view.
PDB entry 1zh4 coloured by chain, side view.
PDB entry 1zh4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A common dimerization interface in bacterial response regulators KdpE and TorR.
Toro-Roman A, Wu T, Stock AM
Protein Sci 14 3077-88 (2005)
PMID: 16322582

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
KDP operon transcriptional regulatory protein KdpE Chains: A, B
Molecule details ›
Chains: A, B
Length: 121 amino acids
Theoretical weight: 13.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21866 (Residues: 1-121; Coverage: 54%)
Gene names: JW5096, b0694, kdpE
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand BEF 2 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 70.912Å b: 126.675Å c: 62.655Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Evolution of two-component signal transduction systems.
Capra et al. (2012)
9 more

4 mentions without citation

A common dimerization interface in bacterial response regulators KdpE and TorR.
Toro-Roman et al. (2005)
3 more