PDBe 1zbb

X-ray diffraction
9Å resolution

Structure of the 4_601_167 Tetranucleosome

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 36-mer (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
Histone H3.2 Chains: A, E, a, e
Molecule details ›
Chains: A, E, a, e
Length: 135 amino acids
Theoretical weight: 15.3 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 2-136; Coverage: 99%)
Histone H4 Chains: B, F, b, f
Molecule details ›
Chains: B, F, b, f
Length: 102 amino acids
Theoretical weight: 11.26 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 2-103; Coverage: 99%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1 Chains: C, G, c, g
Molecule details ›
Chains: C, G, c, g
Length: 129 amino acids
Theoretical weight: 13.98 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 2-130; Coverage: 99%)
Sequence domains:
Histone H2B 1.1 Chains: D, H, d, h
Molecule details ›
Chains: D, H, d, h
Length: 125 amino acids
Theoretical weight: 13.85 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 2-126; Coverage: 99%)
Sequence domains: Core histone H2A/H2B/H3/H4
DNA STRAND 1 (ARBITRARY MODEL SEQUENCE) Chain: I
Molecule details ›
Chain: I
Length: 347 nucleotides
Theoretical weight: 107.15 KDa
DNA STRAND 2 (ARBITRARY MODEL SEQUENCE) Chain: J
Molecule details ›
Chain: J
Length: 347 nucleotides
Theoretical weight: 107.14 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I222
Unit cell:
a: 127.675Å b: 168.445Å c: 237.126Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.386 0.386 not available
Expression system: Escherichia coli