PDBe 1z7h

X-ray diffraction
2.3Å resolution

2.3 Angstrom crystal structure of tetanus neurotoxin light chain

Source organism: Clostridium tetani
Primary publication:
2.3 A crystal structure of tetanus neurotoxin light chain.
Biochemistry 44 7450-7 (2005)
PMID: 15895988

Function and Biology Details

Reaction catalysed:
Hydrolysis of -Gln(76)-|-Phe- bond in synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP).
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Tetanus toxin light chain Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 51.22 KDa
Source organism: Clostridium tetani
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P04958 (Residues: 1-443; Coverage: 34%)
Gene names: CTC_p60, tetX
Sequence domains: Clostridial neurotoxin zinc protease
Structure domains: Metalloproteases ("zincins"), catalytic domain like

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C222
Unit cell:
a: 105.35Å b: 176.86Å c: 57.23Å
α: 90° β: 90° γ: 90°
R R work R free
0.219 0.212 0.216
Expression system: Escherichia coli BL21(DE3)