1z2c

X-ray diffraction
3Å resolution

Crystal structure of mDIA1 GBD-FH3 in complex with RhoC-GMPPNP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-126687 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho-related GTP-binding protein RhoC Chains: A, C
Molecule details ›
Chains: A, C
Length: 193 amino acids
Theoretical weight: 22.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08134 (Residues: 1-193; Coverage: 100%)
Gene names: ARH9, ARHC, RHOC
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Protein diaphanous homolog 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 383 amino acids
Theoretical weight: 44.22 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O08808 (Residues: 69-451; Coverage: 31%)
Gene names: Diap1, Diaph1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 148.64Å b: 85.35Å c: 123.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.285
Expression system: Escherichia coli BL21(DE3)