PDBe 1z25

X-ray diffraction
2.7Å resolution

Structure of P.furiosus Argonaute with bound Mn2+

Source organism: Pyrococcus furiosus
Primary publication:
Purified Argonaute2 and an siRNA form recombinant human RISC.
Nat. Struct. Mol. Biol. 12 340-9 (2005)
PMID: 15800637

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Argonaute Chain: A
Molecule details ›
Chain: A
Length: 771 amino acids
Theoretical weight: 90.6 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
  • Canonical: Q8U3D2 (Residues: 1-770; Coverage: 100%)
Gene name: PF0537
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21
Unit cell:
a: 68.662Å b: 105.108Å c: 73.662Å
α: 90° β: 102.7° γ: 90°
R R work R free
0.26 0.215 0.274
Expression system: Escherichia coli