› 1z1b

X-ray diffraction
3.8Å resolution

Crystal structure of a lambda integrase dimer bound to a COC' core site

Released:
DOI: 10.2210/pdb1z1b/pdb
PDB entry 1z1b coloured by chain, front view.
PDB entry 1z1b coloured by chain, side view.
PDB entry 1z1b coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
A structural basis for allosteric control of DNA recombination by lambda integrase.
Biswas T, Aihara H, Radman-Livaja M, Filman D, Landy A, Ellenberger T
Nature 435 1059-66 (2005)
PMID: 15973401

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114877 (preferred)
Entry contents:
1 distinct polypeptide molecule
5 distinct DNA molecules
Macromolecules (6 distinct):
Integrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 356 amino acids
Theoretical weight: 40.45 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03700 (Residues: 1-356; Coverage: 100%)
Gene name: int
Sequence domains:
Structure domains:
5'-D(*CP*T*CP*GP*TP*TP*CP*AP*GP*CP*TP*TP*TP*TP*TP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.23 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
5'-D(*TP*TP*TP*AP*TP*AP*CP*TP*AP*AP*GP*TP*TP*GP*GP*CP*AP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
29-MER DNA Chain: E
Molecule details ›
Chain: E
Length: 29 nucleotides
Theoretical weight: 8.94 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
26-MER DNA Chain: F
Molecule details ›
Chain: F
Length: 26 nucleotides
Theoretical weight: 7.95 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
26-MER DNA Chain: G
Molecule details ›
Chain: G
Length: 26 nucleotides
Theoretical weight: 8.02 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 97.599Å b: 211.278Å c: 191.704Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.296
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

20 review citations

Mechanisms of site-specific recombination.
Grindley et al. (2006)
19 more

9 mentions without citation

DNA arms do the legwork to ensure the directionality of lambda site-specific recombination.
Radman-Livaja et al. (2006)
8 more