PDBe 1yz9

X-ray diffraction
2.1Å resolution

Crystal structure of RNase III mutant E110Q from Aquifex aeolicus complexed with double stranded RNA at 2.1-Angstrom Resolution


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 26.15 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
  • Canonical: O67082 (Residues: 1-221; Coverage: 100%)
Gene names: aq_946, rnc
Sequence domains:
Structure domains:
5'-R(*CP*GP*AP*AP*CP*UP*UP*CP*GP*CP*G)-3' Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 11 nucleotides
Theoretical weight: 3.48 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 60.586Å b: 118.362Å c: 61.9Å
α: 90° β: 99.45° γ: 90°
R R work R free
0.21 0.208 0.238
Expression systems:
  • Escherichia coli
  • Not provided