PDBe 1yve

X-ray diffraction
1.65Å resolution

ACETOHYDROXY ACID ISOMEROREDUCTASE COMPLEXED WITH NADPH, MAGNESIUM AND INHIBITOR IPOHA (N-HYDROXY-N-ISOPROPYLOXAMATE)

Released:

Function and Biology Details

Reaction catalysed:
(1) (2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2- methyl-3-oxobutanoate + NADPH. (2) (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2- ethyl-3-oxobutanoate + NADPH. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ketol-acid reductoisomerase, chloroplastic Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 524 amino acids
Theoretical weight: 57.05 KDa
Source organism: Spinacia oleracea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01292 (Residues: 72-595; Coverage: 88%)
Gene name: AHRI
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 111.43Å b: 61.94Å c: 162.54Å
α: 90° β: 95.08° γ: 90°
R-values:
R R work R free
0.197 0.197 0.239
Expression system: Escherichia coli