› 1yv4

X-ray diffraction
1.51Å resolution

X-ray structure of M23L onconase at 100K

Released:
DOI: 10.2210/pdb1yv4/pdb
PDB entry 1yv4 coloured by chain, front view.
PDB entry 1yv4 coloured by chain, side view.
PDB entry 1yv4 coloured by chain, top view.
Source organism: Lithobates pipiens
Primary publication:
The importance of dynamic effects on the enzyme activity: X-ray structure and molecular dynamics of onconase mutants.
Merlino A, Mazzarella L, Carannante A, Di Fiore A, Di Donato A, Notomista E, Sica F
J Biol Chem 280 17953-60 (2005)
PMID: 15728177

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein P-30 Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.83 KDa
Source organism: Lithobates pipiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22069 (Residues: 1-104; Coverage: 100%)
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 32.29Å b: 38.544Å c: 69.096Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.156 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Ribonucleases as novel chemotherapeutics : the ranpirnase example.
Lee et al. (2008)
2 more

1 mention without citation

Crystal structure of Onconase at 1.1 Å resolution--insights into substrate binding and collective motion.
Holloway et al. (2011)