PDBe 1ysa

X-ray diffraction
2.9Å resolution

THE GCN4 BASIC REGION LEUCINE ZIPPER BINDS DNA AS A DIMER OF UNINTERRUPTED ALPHA HELICES: CRYSTAL STRUCTURE OF THE PROTEIN-DNA COMPLEX

Released:

Function and Biology Details

Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
General control protein GCN4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 58 amino acids
Theoretical weight: 6.89 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P03069 (Residues: 225-281; Coverage: 20%)
Gene names: AAS3, ARG9, GCN4, YEL009C
Structure domains: Classic Zinc Finger
DNA (5'-D(*AP*AP*AP*CP*TP*GP*GP*AP*TP*GP*AP*GP*TP*CP*AP*TP*A P*GP*GP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.23 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*TP*CP*CP*TP*AP*TP*GP*AP*CP*TP*CP*AP*TP*CP*CP*A P*GP*TP*T)-3') Chain: A
Molecule details ›
Chain: A
Length: 20 nucleotides
Theoretical weight: 6.03 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-13
Spacegroup: P212121
Unit cell:
a: 49.13Å b: 88.54Å c: 59.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 not available not available
Expression system: Not provided