PDBe 1yrn

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF THE MATA1/MATALPHA2 HOMEODOMAIN HETERODIMER BOUND TO DNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Mating-type protein A1 Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 7.16 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CY10 (Residues: 66-126; Coverage: 48%)
Gene names: MAT1A, MATA1
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
Mating-type protein ALPHA2 Chain: B
Molecule details ›
Chain: B
Length: 83 amino acids
Theoretical weight: 9.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CY08 (Residues: 128-210; Coverage: 40%)
Gene names: ALPHA-2, MAT2A, MATAL2, MATALPHA2, YCR039C, YCR39C
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
DNA (5'-D(*TP*AP*CP*AP*TP*GP*TP*AP*AP*TP*TP*TP*AP*TP*TP*AP*C P*AP*TP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.4 KDa
DNA (5'-D(*TP*AP*TP*GP*AP*TP*GP*TP*AP*AP*TP*AP*AP*AP*TP*TP*A P*CP*AP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.48 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P61
Unit cell:
a: 132.38Å b: 132.38Å c: 45.86Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.298
Expression system: Escherichia coli