PDBe 1yrc

X-ray diffraction
1.4Å resolution

X-ray Crystal Structure of hydrogenated Cytochrome P450cam

Released:
Source organism: Pseudomonas putida
Primary publication:
Production and X-ray crystallographic analysis of fully deuterated cytochrome P450cam.
Acta Crystallogr. D Biol. Crystallogr. 61 539-44 (2005)
PMID: 15858263

Function and Biology Details

Reaction catalysed:
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 46.59 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00183 (Residues: 2-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome p450

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43212
Unit cell:
a: 63.65Å b: 63.65Å c: 246.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.219
Expression system: Escherichia coli BL21(DE3)