PDBe 1yov

X-ray diffraction
2.6Å resolution

Insights into the Ubiquitin Transfer Cascade from the refined structure of the activating enzyme for NEDD8

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NEDD8-activating enzyme E1 regulatory subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 537 amino acids
Theoretical weight: 60.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13564 (Residues: 1-534; Coverage: 100%)
Gene names: APPBP1, HPP1, NAE1
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain
NEDD8-activating enzyme E1 catalytic subunit Chains: B, D
Molecule details ›
Chains: B, D
Length: 444 amino acids
Theoretical weight: 49.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TBC4 (Residues: 22-463; Coverage: 96%)
Gene names: UBA3, UBE1C
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2, NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 92.4Å b: 123.6Å c: 198.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.236 0.28
Expression system: Escherichia coli