PDBe 1ynt

X-ray diffraction
3.1Å resolution

Structure of the immunodominant epitope displayed by the surface antigen 1 (SAG1) of Toxoplasma gondii complexed to a monoclonal antibody

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Protein L Chain: E
Molecule details ›
Chain: E
Length: 61 amino acids
Theoretical weight: 6.75 KDa
Source organism: Finegoldia magna ATCC 29328
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51918 (Residues: 476-536; Coverage: 6%)
Structure domains: Ubiquitin-like (UB roll)
Major surface antigen p30 Chains: F, G
Molecule details ›
Chains: F, G
Length: 254 amino acids
Theoretical weight: 26.64 KDa
Source organism: Toxoplasma gondii
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P13664 (Residues: 50-303; Coverage: 88%)
Sequence domains: SRS domain
Structure domains: Major surface antigen p30, SAG1
4F11E12 Fab variable heavy chain region Chains: B, D
Molecule details ›
Chains: B, D
Length: 218 amino acids
Theoretical weight: 23.57 KDa
Source organism: Mus musculus
UniProt:
Structure domains: Immunoglobulins
4F11E12 Fab variable light chain region Chains: A, C
Molecule details ›
Chains: A, C
Length: 213 amino acids
Theoretical weight: 23.58 KDa
Source organism: Mus musculus
UniProt:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 71.034Å b: 198.285Å c: 128.366Å
α: 90° β: 89.97° γ: 90°
R-values:
R R work R free
0.241 0.241 0.283
Expression systems:
  • Escherichia coli
  • Komagataella pastoris