› 1yfi

X-ray diffraction
2.7Å resolution

Crystal Structure of restriction endonuclease MspI in complex with its cognate DNA in P212121 space group

Released:
DOI: 10.2210/pdb1yfi/pdb
PDB entry 1yfi coloured by chain, front view.
PDB entry 1yfi coloured by chain, side view.
PDB entry 1yfi coloured by chain, top view.
Source organism: Moraxella sp.
Primary publication:
Two crystal forms of the restriction enzyme MspI-DNA complex show the same novel structure.
Xu QS, Roberts RJ, Guo HC
Protein Sci 14 2590-600 (2005)
PMID: 16195548

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114861 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type II restriction enzyme MspI Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 29.88 KDa
Source organism: Moraxella sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P11405 (Residues: 1-262; Coverage: 100%)
Gene name: mspIR
Sequence domains: Restriction endonuclease MspI
5'-D(*CP*CP*CP*CP*CP*GP*GP*GP*GP*G)-3' Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Moraxella sp.
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 50.295Å b: 111.266Å c: 130.735Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.226 0.274
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
13 other articles

1 mention without citation

Two crystal forms of the restriction enzyme MspI-DNA complex show the same novel structure.
Xu et al. (2005)