PDBe 1ybq

X-ray diffraction
2Å resolution

Crystal structure of Escherichia coli isoaspartyl dipeptidase mutant D285N complexed with beta-aspartylhistidine

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Isoaspartyl dipeptidase Chain: A
Molecule details ›
Chain: A
Length: 390 amino acids
Theoretical weight: 41.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P39377 (Residues: 1-390; Coverage: 100%)
Gene names: JW4291, b4328, iadA, yjiF
Sequence domains: Amidohydrolase family
Structure domains:
Isoaspartyl dipeptidase Chain: B
Molecule details ›
Chain: B
Length: 390 amino acids
Theoretical weight: 41.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P39377 (Residues: 1-390; Coverage: 100%)
Gene names: JW4291, b4328, iadA, yjiF
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P4212
Unit cell:
a: 119.3Å b: 119.3Å c: 138.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.181 0.243
Expression system: Escherichia coli